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prism–type total internal reflection fluorescence (tirf) microscope  (Nikon)


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    Structured Review

    Nikon prism–type total internal reflection fluorescence (tirf) microscope
    Prism–Type Total Internal Reflection Fluorescence (Tirf) Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+type+total+internal+reflection+fluorescence+tirf+microscope/pm38065943-323-12-20
    Average 90 stars, based on 1 article reviews
    prism–type total internal reflection fluorescence (tirf) microscope - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Structure–activity relationships at a nucleobase-stacking tryptophan required for chemomechanical coupling in the DNA resecting motor-nuclease AdnAB
    Article Snippet: The protein concentrations were determined by using the BioRad dye reagent with BSA as the standard. .. All experiments were performed with a custom-built prism-type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with a 488-nm laser (Coherent Sapphire, 200 mW) and two Andor iXon EMCCD cameras ( ). .. Flowcells and single-tethered dsDNA curtains were prepared as described previously ( ) using bacteriophage λ DNA (48.5 kb; NEB Cat No. N3011S) that was biotinylated at one end and anchored to a lipid bilayer through a biotin-streptavidin linkage.

    Article Title: Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments
    Article Snippet: .. TIRFM experiments – All experiments were conducted with a custom-built prism type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with 488 nm laser (Coherent Sapphire, 200 mW) and a 561-nm laser (Coherent Sapphire, 200 mW), as previously described (28,29). ..

    Article Title: Single-molecule visualization of human RECQ5 interactions with single-stranded DNA recombination intermediates
    Article Snippet: .. All single molecule experiments were conducted with a prism-type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with a 488-nm laser (Coherent Sapphire, 200 mW), a 561-nm laser (Coherent Sapphire, 200 mW), and two Andor iXon EMCCD cameras ( , ). .. The microscope was equipped with a 60x Nikon objective lens (N.A.

    Article Title: Dynamic interactions of the homologous pairing 2 (Hop2)–meiotic nuclear divisions 1 (Mnd1) protein complex with meiotic presynaptic filaments in budding yeast
    Article Snippet: .. TIRFM experiments – All experiments were conducted with a custom-built prism type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with 488 nm laser (Coherent Sapphire, 200 mW) and a 561-nm laser (Coherent Sapphire, 200 mW), as previously described (49,74,75). ..

    Article Title: Biophysical characterization of the structural change of Nopp140, an intrinsically disordered protein, in the interaction with CK2α
    Article Snippet: All measurements of single molecule experiments were carried out in smFRET buffer (20 mM Tris-Cl, pH 7.4; 250 mM NaCl) containing an oxygen scavenging system (2 mM Trolox (Sigma, USA), 0.8% (w/v) D-glucose (Sigma, USA), 165 U/ml glucose oxidase (Sigma, USA), 2170 U/ml catalase (Sigma, USA) [ 10 ]. .. The single molecule signals of donor (Cy3) and acceptor (Cy5) were acquired in a prism-type total internal reflection fluorescence (TIRF) microscope (Nikon, Ti-U, water-type 60× objective, NA = 1.2) by an electron multiplying charged-coupled device (Andor iXon EM +897) at 50 ms time resolution. .. The signals were filtered through532 nm and 633 nm notch filters (NF03-532E-25 and NF01-633U-25; Semrock) and separated using DV2 (Photometrics, with T660lpxr, Chroma Technology).

    Article Title: The RecQ helicase Sgs1 drives ATP-dependent disruption of Rad51 filaments
    Article Snippet: .. All experiments were conducted with a prism-type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with a 488-nm laser (Coherent Sapphire, 200 mW), a 561-nm laser (Coherent Sapphire, 200 mW), and two Andor iXon EMCCD cameras ( , ). ..

    Microscopy:

    Article Title: Structure–activity relationships at a nucleobase-stacking tryptophan required for chemomechanical coupling in the DNA resecting motor-nuclease AdnAB
    Article Snippet: The protein concentrations were determined by using the BioRad dye reagent with BSA as the standard. .. All experiments were performed with a custom-built prism-type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with a 488-nm laser (Coherent Sapphire, 200 mW) and two Andor iXon EMCCD cameras ( ). .. Flowcells and single-tethered dsDNA curtains were prepared as described previously ( ) using bacteriophage λ DNA (48.5 kb; NEB Cat No. N3011S) that was biotinylated at one end and anchored to a lipid bilayer through a biotin-streptavidin linkage.

    Article Title: Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments
    Article Snippet: .. TIRFM experiments – All experiments were conducted with a custom-built prism type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with 488 nm laser (Coherent Sapphire, 200 mW) and a 561-nm laser (Coherent Sapphire, 200 mW), as previously described (28,29). ..

    Article Title: Single-molecule visualization of human RECQ5 interactions with single-stranded DNA recombination intermediates
    Article Snippet: .. All single molecule experiments were conducted with a prism-type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with a 488-nm laser (Coherent Sapphire, 200 mW), a 561-nm laser (Coherent Sapphire, 200 mW), and two Andor iXon EMCCD cameras ( , ). .. The microscope was equipped with a 60x Nikon objective lens (N.A.

    Article Title: Dynamic interactions of the homologous pairing 2 (Hop2)–meiotic nuclear divisions 1 (Mnd1) protein complex with meiotic presynaptic filaments in budding yeast
    Article Snippet: .. TIRFM experiments – All experiments were conducted with a custom-built prism type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with 488 nm laser (Coherent Sapphire, 200 mW) and a 561-nm laser (Coherent Sapphire, 200 mW), as previously described (49,74,75). ..

    Article Title: Biophysical characterization of the structural change of Nopp140, an intrinsically disordered protein, in the interaction with CK2α
    Article Snippet: All measurements of single molecule experiments were carried out in smFRET buffer (20 mM Tris-Cl, pH 7.4; 250 mM NaCl) containing an oxygen scavenging system (2 mM Trolox (Sigma, USA), 0.8% (w/v) D-glucose (Sigma, USA), 165 U/ml glucose oxidase (Sigma, USA), 2170 U/ml catalase (Sigma, USA) [ 10 ]. .. The single molecule signals of donor (Cy3) and acceptor (Cy5) were acquired in a prism-type total internal reflection fluorescence (TIRF) microscope (Nikon, Ti-U, water-type 60× objective, NA = 1.2) by an electron multiplying charged-coupled device (Andor iXon EM +897) at 50 ms time resolution. .. The signals were filtered through532 nm and 633 nm notch filters (NF03-532E-25 and NF01-633U-25; Semrock) and separated using DV2 (Photometrics, with T660lpxr, Chroma Technology).

    Article Title: The RecQ helicase Sgs1 drives ATP-dependent disruption of Rad51 filaments
    Article Snippet: .. All experiments were conducted with a prism-type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with a 488-nm laser (Coherent Sapphire, 200 mW), a 561-nm laser (Coherent Sapphire, 200 mW), and two Andor iXon EMCCD cameras ( , ). ..

    other:

    Article Title: Biophysical characterization of the structural change of Nopp140, an intrinsically disordered protein, in the interaction with CK2α
    Article Snippet: The signals were filtered through532 nm and 633 nm notch filters (NF03-532E-25 and NF01-633U-25; Semrock) and separated using DV2 (Photometrics, with T660lpxr, Chroma Technology).



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    KEY RESOURCES TABLE
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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: Rad52 Restrains Resection at DNA Double-Strand Break Ends in Yeast

    doi: 10.1016/j.molcel.2019.08.017

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: All experiments were conducted with a custom-built prism-type total internal reflection fluorescence (TIRF) microscope (Nikon) equipped with a 488 nm laser (Coherent Sapphire, 200 mW) and a 561 nm laser (Coherent Sapphire, 200 mW)( De Tullio et al., 2018 ; Ma et al., 2017 ).

    Techniques: Produced, Recombinant, Protease Inhibitor, Polymer, Cloning, DNA Labeling, SYBR Green Assay, Western Blot, Fluorescence, Microscopy, Software, Real-time Polymerase Chain Reaction, Imaging, Hybridization, Membrane